Comparison of Two Antigens for Diagnosis of Trichinellosis in Pigs
نویسندگان
چکیده
Due to its role in human Trichinellosis, there are increasing global requirements for reliable diagnostic method for Trichinella spiralis infection in pigs. Consequently, there is a need for selection of potent diagnostic antigen that could be successfully utilized in the diagnosis. Currently, two antigens (somatic and excretory-secretory antigens of T. spiralis larvae) were evaluated for the diagnosis of pig trichinellosis using ELISA. The ES antigen exhibited highest diagnostic potential (88.3%) as compared to somatic antigen (68.3%). The diagnostic potential of ES antigen is attributed to five immunogenic bands identified by infected pig sera in immunoblot. These bands are of molecular weight 127, 58, 35, 20.5 and 12 KDa. While in somatic antigen six immunogenic bands of molecular weight 230, 89, 58, 27, 15 and 6 KDa were identified. In conclusion, monitoring for T. spiralis antibodies in swine farms should be based on screening pig sera by means of ELISA in which larval ES antigen was utilized.
منابع مشابه
Trichinella britovi as a risk factor for alternative pig production systems in Greece and Europe
Trichinellosis is an important zoonosis and the most common source of human infection is meat from pigs and wild boars. The European Union (EU) supports alternative pig production systems (organic and free-ranging) as sustainable farming systems. However, these systems that allow outdoor access for farm animals, may create new or reintroduce old risks to public health. During the last years, al...
متن کاملComparison of two antigens for demonstration of Trichinella spp. antibodies in blood and muscle fluid of foxes, pigs and wild boars.
For the surveillance of trichinellosis, the digestion method is reliable but also labour intensive. The serological methods for the detection of Trichinella-specific antibodies using ELISA offer a sensitive and relatively specific alternative. For serological studies, sera or plasma from blood samples are the most common source of antibodies, but although the concentration of antibodies is appr...
متن کاملEnzyme-linked immunosorbent assay for detection of Trichinella spiralis antibodies and the surveillance of selected pig breeding farms in the Republic of Korea.
Trichinellosis is a parasitic zoonosis of public health importance. It is caused by Trichinella spiralis which has a wide host range including humans. In the present communication, the ELISA technique was employed on a total of 803 blood samples from 7 selected pig breeding farms in 1996 for diagnosis and surveillance of trichinellosis. Out of the entire 803 samples, nine were found to be suspe...
متن کاملIn vitro Lymphoproliferative Responses of Trichostrongylus colubriformis High and Low Responder Guinea Pigs to Worm Antigens (SPL3, SPA) and Ovalbumin
In vitro lymphocyte responses of high responder (HR) and low responder (LR) guinea pigs from pe-ripheral blood lymphocytes (PBL) to parasite antigens soluble protein third stage larvae (SPL3) and soluble protein adult stage ( SPA), non-parasite antigen ovalbumin (OVA) were examined. There was substantial differences between HR and LR guinea pigs in the rate of acquisition of responsiveness to t...
متن کاملComparison of three antigen preparations to detect Trichinellosis in live swine using IgG-ELISA.
A swine infected with Trichinella spiralis is a source of transmission to human through consumption of raw or improperly cooked pork. Detection of larvae is suitable for carcasses, so that pigs in households or farms can be examined serologically for trichinellosis. This study compared antigens, crude (CAg), excretory-secretory (ESAg) and surface (SAg), for their potential use in IgG-ELISA. Ser...
متن کامل